Acetal linkage between the aglycon and glycone more unstable than that between two individual sugars within the molecule. A solution of glycosides is treated with a small amount of kedde reagent mix equal volumes of a 2% solution of 3, 5 dinitrobenzoic acid in menthol and a 7. Hcl for 10 min, filter and shake with organic solvent ether or benzene. All glycosides are hydrolysable by acids except c glycosides. Usa home product directory biochemicals and reagents antibiotics chemical structure class coumarin glycosides life science home life science products. For c types of anthraquinone glycosides i modified brontragers test. A blue, green, red or purple color is a positive test. Physicochemical properties of glycosides colorless, solid, amorphous, nonvolatile flavonoid yellow, anthraquinonered or orange. Test for glycosides a small amount of alcoholic extract was taken in 1 ml of water in a test tube and a few drops of aqueous naoh. Different tests are done because of the different chemical entities of the aglycone moiety. It is a semi quantitative method to find out the presence of sugars that is polysaccharides. Test for phenols a small amount of the ethanolic extract was taken with 1 ml of water in a test tube and 1 to 2 drops of iron iii chloride fecl 3 was added. Physical and chemical properties colourless or white crystals rarely amorphous solubility is determined by the number of sugar moieties.
Take a piece of lamina or thick section of the leaf and add sodium picrate reagent. We have to hydrolyze the drug and then chemical tests are carried out. A purple colour is presence of to an aqueous solution 20ml cuso4 slo 1 drop is formed isobarbaloin aloe added followed by nacl 1g and 90% alcohol 10ml. Give positive reaction with molischs and fehlings solution test after hydrolysis. The procedure for the isolation of glycosides also varies. If glycoside is present yellow to orange colour will be seen. Glycosides chemical properties purvi kakrani, glycosides, lbriper 9 acid hydrolysis.
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